Oligonucleotide cyclization: the thiol-maleimide reaction revisited.
نویسندگان
چکیده
A novel method to synthesize cyclic oligonucleotides (5- to 26-mer) using the thiol-maleimide reaction is described. The target molecules were obtained after subsequent removal of thiol and maleimide protecting groups from 5'-maleimido-3'-thiol-derivatized linear precursors. Retro-Diels-Alder conditions deprotecting the maleimide simultaneously promoted cyclization cleanly and in high yield.
منابع مشابه
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ورودعنوان ژورنال:
- Chemical communications
دوره 49 3 شماره
صفحات -
تاریخ انتشار 2013